Newsletter - Annals of the Brazilian Academy of Sciences

ISSUE 58

AABC - volume 94.4 2022

The present issue of the Annals of the Brazilian Academy of Sciences (AABC) has a total of 67 articles and one letter, covering almost all scientific fields published by this journal.

The largest number of contributions in this issue come from the area of Animal Science (AnSci - 18), followed by Ecosystems (Ecosy - 10), Cellular and Molecular Biology (CeMoBio - 8), Engineering Sciences (EnSci - 7), and Crop Science (CrSci - 6). There are also contributions in Health Sciences (HeSci - 4), Biomedical Sciences (BmSci - 3), Microbiology (Mcbio - 3), Geosciences (Geosci - 3), Soil Science (SoiSci - 1), Social Sciences (SocSci - 1), Mathematical Sciences (MaSci - 1), Paleontology (Paleo - 1), and Chemical Sciences (ChSci - 1).

Highlights are always hard to pick. In Animal Science there are studies on helminth endoparasites in fish from Minas Gerais, pathologies in turtle eggs, nutrition of goats in different stages of pregnancy, and a scientometric analysis regarding the scientific literature on bats.
Engineering Sciences provide studies on the eutrophication risks of a large reservoir in the semiarid region of Brazil, assessment of water quality using a model based on remote sensing, and the evaluation of the effectiveness of power plants in Russia.

Other contributions deal with a report on the venom of the most venomous fish found on the Brazilian coastal area (CeMoBio), insect galls from a reserve in São Paulo (Ecosy), radiotherapy protocols for lung cancer (BmSci), and a novel enzymatic extract from Bromelia (ChSci).
I would also like to draw your attention to the editorial, which provides some brief comments on the contributions of articles on COVID-19 published by the AABC since the beginning of the pandemic.

Please keep in mind that since 2000 all papers published by the AABC can be downloaded free of charge at the SciELO site and previous editions of the Newsletter are available at the ABC website.

We are now inviting you to scroll through the text and click on the title of the article that interests you!

Alexander W. A. Kellner
Editor-in-Chief

MATHEMATICAL SCIENCES

1- A New Class of Exponentiated Beta-Skew-Laplace Distribution

ROGER TOVAR-FALÓN & GUILLERMO MARTÍNEZ-FLÓREZ

This paper introduce two new families of distributions that allow fitting unimodal, bimodal or trimodal data sets. Statistical properties such as distribution function, moments, moment generating function and stochastic representation of these new families are studied in details. The problem of estimating parameters is addressed by considering the maximum likelihood method and Fisher information matrices are derived. A small Monte Carlo simulation study is conducted to examine the performance of the obtained estimators. The methodology developed is illustrated with three real data applications 

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CHEMICAL SCIENCES

2- Proteolytic characterization of a novel enzymatic extract from Bromelia serra leaves

MELANIE D. GÓMEZ HERRERA, PAULA ALAYÓN LUACES, CONSTANZA LIGGIERI, MARIELA BRUNO & MARÍA VICTORIA AVANZA

Enzymes are biocatalysts that are employed in several industrial commercial applications. The majority of proteases studied in Bromeliaceae family come from their fruits. Nevertheless, the presence of these fruits depends on the climate or season to bloom. The availability of leaves is constant and in the case of Bromelia serra (BS) is abundant. The aim of this work was to prepare an extract from leaves of BS, analyze and characterize the proteolytic system present in this plant material. The knowledge about the enzymatic extract characteristics may be useful as it can be applied as a catalyst in different industrial areas. 

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BIOMEDICAL SCIENCES

3- Cellular bases of hypofractionated radiotherapy protocols for lung cancer

ELIANA EVELINA OCOLOTOBICHE, YULIANA CATALINA BANEGAS, GUSTAVO FERRARIS, MARCELO MARTÍNEZ & ALBA MABEL GÜERCI

The healthcare demand during the COVID-19 pandemic led to the reconsideration of empirically applied hypofractionated radiotherapy protocols. In an attempt to determine the cellular and molecular basis of these procedures, we evaluated the genotoxic, cytotoxic, proliferative and clonogenic effects of conventional and hypofractionated radiation doses on A549 cell cultures and their respective conditioned media. The greater efficacy of ablative regimens was demonstrated and attributed both to the direct effects of radiation and to the indirect action of clastogenic factors secreted into the medium, thus establishing a multifactorial mechanism of action that exceeds the 4Rs of conventional radiotherapy. 

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4- Hederagenin Exerts Potential Antilipemic Effect via p38MAPK Pathway in Oleic Acid-induced HepG2 cells and in Hyperlipidemic Rats

MENG YANG, JING WANG & QIAOLING WANG

This manuscript is the first to study the effect on lipid-lowering of hederagenin in vivo and in vitro. In vitro, TG was used as the index to verify the lipid-lowering effect of hederagenin in oleic acid-induced HepG2 cells. In vivo, TC, TG, LDL-C, HDL-C MDA, SOD, and GSH-PX were used as direct indicators to study the antilipemic and anti-oxidative effect of hederagenin. The signaling pathways of anti-oxidation were evaluated using Western blot. The results showed that hederagenin has a good effect of lowering blood lipids, which might be related to the antioxidant mechanism of MAPK pathway.

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5- Cytotoxicity, antinociceptive and gastroproiltective potential of the Abuta selloana Eichler: a fruit plant from Catarinense flora, Brazil

LUCIANE A.N. NESELLO, ADRIANA CAMPOS, ANDRÉA REBELLO, FRANCIELLI T. MOTTA, LUISA N.B. MARIANO, FÁTIMA C. BUZZI, ANA LÚCIA T.G. RUIZ, JOÃO ERNESTO DE CARVALHO, VALDIR CECHINEL-FILHO & LUISA M. SILVA

Our study evaluated some biological activities of extracts from Abuta selloana. We observed higher levels of phenolic compounds in all extracts, which can justify the in vitro antioxidant activity of all extracts. Besides, only the extract from leaves presented potent cytotoxicity against tumoral cells; whereas the extracts from fruits, branches and leaves reduced pain behavioral and prevented the gastric ulcer in rodents. In conclusion, A. selloana had interesting biological activities, presenting the leaves as a promising source for compounds with cytotoxic potential. Moreover, the whole plant can be an important source of bioactive compounds associated with gastroprotective and analgesic properties. 

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HEALTH SCIENCES

6- Comparison of safety and efficacy of convalescent plasma with fresh frozen plasma in severe COVID-19 patients

MEENU BAJPAI, ASHISH MAHESHWARI, SURESH KUMAR, KARAN CHHABRA, PRATIBHA KALE, ASHAD NARAYANAN, AMITA GUPTA, EKTA GUPTA, NIRUPAMA TREHANPATI, RESHU AGARWAL, KAMINI GUPTA, ANKIT BHARDWAJ, MOJAHIDUL ISLAM, RAVINDER SINGH, PUSHPA YADAV, GURESH KUMAR & SHIV K. SARIN

This manuscript highlights the role of Convalescent Plasma Therapy compared to Fresh Frozen Plasma in the severely Sick COVID-19 Patients. It is the first registered randomized control trial conducted in the severely sick COVID-19 patients where we compared the convalescent plasma (from recovered COVID-19 donors) with fresh frozen plasma (collected from the healthy blood donor before the era of COVID-19 to counter the beneficial effects of plasma with its coagulation factors) to see the efficacy of convalescent plasma in terms of mortality and hospital stay in patients who were transfused along with standard medical treatment. 

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7- Histomorphometric analysis of the lung of Swiss mice treated with a fibrinolytic protease

MARLLYN M. DA SILVA, MARIA A.C. DE LIRA, TAMIRIS A. ROCHA, DANIELLE F. DE MOURA, FRANCISCO C.A.A. JÚNIOR, ANA V.S. FERREIRA, LORENZO PASTRANA, WENDELL W.C. ALBUQUERQUE, ROMERO M.P.B. COSTA, THIAGO P. NASCIMENTO & ANA L.F. PORTO

Fibrinolytic enzymes are considered a promising alternative in the treatment of cardiovascular diseases. In this manuscript, a fibrinolytic protease produced by the fungus Mucor subtilissimus UCP 1262 and purified by chromatography was administered at an acute dose of 2000 mg/mL to evaluate its toxic effects on the lungs of mice. After 14 days of treatment, a histomorphometric study was performed by counting type 1 and 2 pneumocytes and evaluating the lung area. There was a decrease in type 2 pneumocytes and significant pulmonary non-toxicity, emphysema and fibrosis were detected. Thus, in vitro tests suggest possible applications of the enzyme. 

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8- Acute toxicity and regenerative dose finding of an extract of Miconia ferruginata DC. in a mouse model of Duchenne muscular dystrophy

PAULA M. MUNNO, POLIANA R. BARROSO, BRUNA F. VASCONCELOS, GEOVANNE B.F. DA SILVA, THIAGO M. SALGUEIRO, HELOISA H. AGUIAR, LETÍCIA M. VITORINO, MURILO X. OLIVEIRA, HELEN R. MARTINS, THAIS P. GAIAD & ALEX S.D. MACHADO

Duchenne muscular dystrophy is severe inflammatory disease with no cure. M. ferruginata showed promisor antiinflammatory effects in vitro, however its toxic or therapeutic dose was unknown. This study demonstrated LD50 of the aqueous extract of M. ferruginata leaves in mdx mice was 1000 mg kg^-1. No toxicity was found in 200, 100, and 50 mg kg^-1 doses. The 50 mg kg^-1 dose showed promisor antiinflammatory effects in vivo, reduced fibrosis, cell stress and increased anti-apoptotic MCL1. M. ferruginata extract present a promising use in dystrophic muscles. 

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9- Antioxidant and anti-inflammatory activity of curcumin transdermal gel in an IL-10 knockout mouse model of inflammatory bowel disease

FERNANDA O. ARAUJO, MATHEUS B. FELÍCIO, CÁSSIO F. LIMA, MAYRA S. PICCOLO, VIRGÍNIA R. PIZZIOLO, GASPAR DIAZ-MUÑOZ, DANIEL S.S. BASTOS, LEANDRO L. OLIVEIRA, MARIA DO C.G. PELUZIO & MARISA A.N. DIAZ

Inflammatory bowel diseases (IBD) are a group of inflammatory disorders of the gastrointestinal tract. Crohn’s disease and ulcerative colitis are the main types of IBD. Their prevalence is still low in Brazil, but the incidence is increasing annually. Curcumin have been shown to reduce oxidative stress and aid in the prevention of associated diseases. This study aimed to assess the effect of curcumin transdermal gel on oxidative stress and intestinal inflammation in IL-10 knockout mice. The results demonstrates the protective effects of curcumin transdermal gel on oxidative stress and its potential as adjunctive therapy in IBD. 

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CELLULAR AND MOLECULAR BIOLOGY

10- Germination and in vitro development of mature zygotic embryos and protein profile of seedlings of wild and cultivated Hevea brasiliensis

JOSIANE C. DE CARVALHO, GLEISSON DE O. NASCIMENTO, ANA CLAUDIA L. DA SILVA, MARIA DAS GRACAS R. FERREIRA, WAGNER L. ARAÚJO & JOSÉ FRANCISCO DE C. GONÇALVES

This study compares the germination and development of mature zygotic embryos (MZE) as well as in vitro proteins profile between two genotypes of Hevea brasiliensis, wild and clone (PB 250). Plant establishment was examined and different interactions using the plant growth regulators Indole 3-Acetic Acid (IAA) and 6-Benzylaminopurine (BAP) were investigated. Both genotypes showed higher germination and establishment, and the proteins were characterized in bands ranging from 7 to 30 kDa. Remarkably, the wild-type genotype showed bands of 14 kDa, which may be associated with latex synthesis proteins. Our findings contribute to the understanding of the protein dynamics in different rubber tree genotypes. 

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11- Preventive effects of fixed and progressive forced exercises on memory and brain electrical activity in morphine-addicted rats

NILOOFAR ASHTARI, MARYAM RADAHMADI & HOJJATALLAH ALAEI

Exercise and drugs like morphine influence brain functions. Morphine strongly reflected changes in brain electroencephalography (EEG) as a cellular mechanism. Progressive exercise improved learning and memory but reduced locomotor activity. Whereas fixed exercise affected EEG in the addicted subjects. Clearly, different (fixed or progressive) exercise models produced different changes in brain functions. The fixed exercise was found capable of increasing only the alpha wave power involved in enhancing semantic information processing in addicted subjects. Finally, compared to running started at a high speed, exercise started at a low speed was found to lead to more losses of body weight in addicted subjects.  

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12- Preliminary report on the hemagglutinating activity of the Scorpaena plumieri fish venom

HELENA B. FIOROTTI, THIAGO G. SOARES, MÁRCIA H. BORGES, ALESSANDRA MATAVEL, FABIANA V. CAMPOS & SUELY G. DE FIGUEIREDO

Many venomous fish species are distributed in marine and freshwater environments around the world and are commonly associated with human injuries. One of the most venomous fish in the Brazilian coast is the scorpionfish Scorpaena plumieri. The venom of this species contains several bioactive molecules that display cytolytic, enzymatic, inflammatory, lethal and hemagglutinating activities, which can affect vital biological systems. The fraction that presented hemagglutinating activity was also associated to the cytolytic and antibacterial activities. The exploration of the potential contained in fish venoms can further the development of new drugs and the understanding of complex physiological processes. 

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13- Embryogenic cultures and somatic embryos development from mature seeds of jabuticaba (Plinia cauliflora (Mart.) Kausel)

FABRÍCIA LORRANE R. OLIVEIRA, BRUNO FRANCISCO SANT’ANNA-SANTOS, HUGO P.F. FRAGA, JULIANA DEGENHARDT & MARGUERITE QUOIRIN

The study aimed at investigating somatic embryogenesis as a propagation method for Plinia cauliflora using seeds as explants. This species is difficult to propagate vegetatively and has recalcitrant seeds, thus micropropagation protocols are viable. This study reports, for the first time, the first steps of Plinia cauliflora somatic embryogenesis using mature seeds as initial explants. Morphoanatomical analyses were combined with somatic embryogenesis protocol development at each phase of the process. It is important to emphasize that this study is the beginning of the development of a somatic embryogenesis process for a native Brazilian woody species, importante for its fruit production. 

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14- Cloning and Expression of β-(1,3-1,4) Glucanase (Lichenase) Gene in Bacillus subtilis RSKK246 to create new Probiotic in aquaculture

ÖZGEN A. ÇAM, MAKBULE BAYLAN & GAMZE MAZI

In this study, recombinant vector pNW33N carrying the β-(1,3-1,4) glucanase (lichenase) gene of Streptococcus bovis genome was transferred to Bacillus subtilis RSKK246 (CMCase+) strain by electroporation. Then, electrotransformation was performed on LB-agar plates containing lichenan.   On the other hand, the protein band corresponding to 26 kDa of the recombinant enzyme was observed by zymogram analysis. These results indicate that the β-(1,3-1,4) glucanase gene has been successfully expressed to the B. subtilis strain RSKK246.  As a result, experiments can be made by adding newly created recombinant bacteria (B.subtilis RSK246/pNW33NLic) as a probiotic feed additive to the rations of fish and other farm animals. 

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15- Morphometric Analysis and Synopsis of Pseudognaphalium (Gnaphalieae, Asteraceae) in North America

SUSANA E. FREIRE, MARIANA A. GROSSI, NÉSTOR D. BAYÓN & CLAUDIA MONTI

PseudognaphaliumKirp. (Angiosperm, Asteraceae, Gnaphalieae) consist of about 60 species mainly distributed in South, Central, and North America. The genus is mainly characterized by its herbaceous habitat, capitula in corymbs or panicles, phyllaries with divided stereome, truncate style branches with apical sweeping hairs, achenes glabrous or short pilose, and free pappus bristles. As a first contribution toward a comprehensive taxonomic review of Pseudognaphalium, we perform the first morphometric analysis of North American species, using UPGMA method for the construction of the dendrogram. Thirty-seven species are recognized, a synopsis including a key to identify species and their associated synonymy is provided.  

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16- Anther structure and pollen development in species of Rubiaceae and anatomical evidence of pathway to morphological dioecy

MARINA D. JUDKEVICH, ROBERTO M. SALAS & ANA M. GONZALEZ

Anther structure and pollen development in species of Rubiaceae and anatomical evidence of pathway to morphological dioecy, is a contribution in which classical plant anatomy is used to understand different aspects related to the reproduction of a selected group of species of the Rubiaceae family. Our data provide information on anther and pollen morphology, pollen dispersal units, and pollen presentation mechanisms. In addition, the presence of orbicules and a new type of placentoid are described.  Finally, we propose an evolutionary sequence of changes towards the reduction of non-functional anthers in Rubiaceae. 

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17- Effect of combining Zingiber officinale and Juglans regia extracts on Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis: antibiofilm action and low toxicity

LEONARDO A. DA SILVA, LUCAS P. RAMOS, TATIANE A. SILVA, SIMONE A.B. DE LAPENA, CARLOS EDUARDO R. SANTOS, AMJAD A. HASNA, ADRIANO BRESSANE & LUCIANE D. DE OLIVEIRA

Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus compound the microbiome of the human epidermis, but disturbances in the microbial balance can lead to skin infections.  The objective was evaluate the activity of the Juglans regia and Zingiber officinale extracts, associated and isolated, applied on planktonic cultures, monotypic and heterotopic biofilms by Skin bacterias. In addition was evaluate the cytotoxicity by herbal medicines on mouse macrophages (Raw 264-7).Results show that  combinations by extracts promoted 84% reduction in heterotypic biofilms and promoted 14% of toxicity on macrophages, concluded to be a possible therapeutic alternative to skin infection.

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MICROBIOLOGY

18- Microbial inoculation and fertilizer application on growth of cowpea and spore-based assemblages of arbuscular mycorrhizal fungi in its rhizophere

INGRID A.N. LINO, DANIELLE K.A. DA SILVA, LINDETE M.V. MARTINS, LEONOR C. MAIA & ADRIANA M. YANO-MELO

Bioinputs use in agriculture has expanded and public policies have encouraged the formulation and application of organisms that can increase plant productivity and protection. Arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria (NFB) make up the group of mutualistic symbionts that bring nutritional benefits, increasing plant tolerance under unfavorable conditions. Although the combined use of these symbiotes can potentially bring synergism in nutritional and non-nutritional benefits, the combinations of NFB and AMF isolates are not always favorable. In this study these aspects are discussed in order to contribute to the safe choice of bioinputs.  

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19- A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation

KESSIA P.S. SOUZA, MÁRCIA N.C. CUNHA, JUANIZE M.S. BATISTA, VAGNE M. OLIVEIRA, THIAGO P. NASCIMENTO, AMANDA E.S. CONNIFF, ROMERO M.P.B. COSTA, TATIANA S. PORTO, CAMILA S. PORTO & ANA LÚCIA F. PORTO

This work aimed to compare the production of collagenolytic proteases produced by M. subtilissimus in submerged fermentation (SF) and solid-state fermentation (SSF) as well as extracting in aqueous two-phase system. Enzyme production was performed in using MS-2 culture medium (SF) and soybean bran as substrate (SSF).  In SSF the highest protease and collagenolytic activities were 362.66 U/mL and 179.81 U/mL, respectively. When compared with SF (26.33 and 18.70 U/mL) higher values were obtained in the activities. Thus, SSF shows promise as a bioprocess for extracellular production of collagenolytic proteases, using of soybean bran as substrate had used sustainable raw material.  

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20- Biodiversity of wild Lactococcus lactis and their geo-spatial relationship with the environment

MARIO M. MAIDANA, FÉLIX I. CONTRERAS & OLGA M. VASEK

The objective of this work was to establish associations between the phenotypic characteristics of wild Lactococcus lactis subsp. lactis isolated from artisanal cheese of Corrientes (Argentina), with climatological, soil and, landscapes characteristics. The strains were space-located, flood-drought events and soil composition data, were used. Cluster analysis included three conglomerates, differentiating strains from soils coming from "flooded landscapes" and those from "sandy hills landscape". The main components highlighted the preference of strains from flooded landscapes by a saline-alkaline environment and, strains from sandy hills landscape by a low medium in salts and acid soil.

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ECOSYSTEMS

21- First record of Tmesiphantes Simon, 1892 (Araneae, Theraphosidae) in Peru: a new species and its phylogenetic placement

MICAELA NICOLETTA, NELSON E. FERRETTI, JUAN C. CHAPARRO & RICK C. WEST

In this work we register for the first time a genus of small-sized tarantulas Tmesiphantes. Also, we describe a new species for this genus and present a phylogenetic hypothesis of its position and relation with the other species. Finally, we present an updated map with the known geographic distribution of all species. 

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22- Look at the trees: synopsis of Terminalia s.s. in the Brazilian Amazon with conservation and distribution implications

RAYANE T.M. RIBEIRO, MARIA I.B. LOIOLA & MARGARETH F. DE SALES

The floristic biodiversity of the Amazon is a target of interest for research with different approaches. However, the basis for knowledge about Amazonian plants requires the survey and recognition of species from different Amazonian ecosystems, especially trees, such as Terminalia (Combretaceae). This is a group of trees with wide occurrence in the Amazon and that show great morphological variation. The synopsis presented here is, therefore, a fundamental subsidy for applied research and actions for the conservation and management of the Amazonian flora.

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23- Microcrustaceans in rice fields: A scientometric analysis from 1977 to 2019

MAIBY GLORIZE DA S. BANDEIRA, KAROLINE P. MARTINS, CLEBER PALMA-SILVA, FABIANA G. BARBOSA, LUIZ U. HEPP & EDÉLTI F. ALBERTONI

We evaluated the worldwide trends in studies of the active and dormant forms of microcrustaceans in rice fields, and the potential of this environment as a stock of diversity through a scientometric analysis. The identified studies were from 18 countries. We quantified 301 species from six groups of microcrustaceans. Of the studies, 87% were centered on the active forms of microcrustaceans rather than dormant forms. We found that 15.5% of the countries that grow rice have identified the richness of microcrustaceans, and even though they are artificial environments, rice fields have high potential to store a high diversity of microcrustaceans. 

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24- First record on enchytraeids in a Savanna Tall Woodland (Cerradão) and Upper Montane Atlantic Forest in Brazil

DOUGLAS ALEXANDRE, CINTIA C. NIVA, ANGELA P. BUSSINGER, ROBÉLIO MARCHÃO, ALCIDES GATTO, RENATA G. DA SILVA, RÜDIGER MARIA SCHMELZ & OSMAR KLAUBERG-FILHO

Enchytraeids are small organisms morphologically and taxonomic related to earthworms, they are known to be an important group of soil invertebrates, involved in process like nutrient cycling and soil formation, but despite their ecological relevance few studies on their biology and ecology have been carried out in subtropical regions. We present a pioneer study on abundance and genus distribution of enchytraeids in four hitherto neglected vegetation types in the biomes of Cerrado and Atlantic Forest. Our results highlight differences in genera composition in each site studied and that the community structure can be influenced by local conditions and land use. 

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25- Diversity, phenology and voltinism of Chironomidae (Diptera). Neotropical streams as a study model

JUAN PABLO ZANOTTO ARPELLINO, LUDMILA NOELIA SOLEDAD RODRÍGUEZ CATANZARO, LUCIANA MONTALTO, AUGUSTO SIRI & MARIANO DONATO

Studies of the relationships between the temporal variation of biological phenomena and environmental factors are essential to understanding community dynamics. This study analyses the composition and structure of the dipteran insect Chironomidae in Neotropical streams and its adult emergence patterns related to environmental variables. Among the streams, the Chironomidae emergence pattern was determined by rainfall, photoperiod, and water and air temperature. The results showed that the different subfamilies emerge in different season. Regarding voltinism, the species in study exhibited mostly one cycle for a year and to a lesser extent two or more cycles. 

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26- Trap and bait efficiency for catching Calliphoridae and Mesembrinellidae (Insecta, Diptera) at different heights

BRUNA LETÍCIA B. FAÇANHA, MARIA CRISTINA ESPOSITO & LEANDRO JUEN

In this manuscript, we evaluated the effectiveness of Calliphoridae and Mesembrinellidae fly collection traps with baits of different natures, one of animal and one of plant origin, decomposing and exposed at two different heights. Flies are a group with ecological importance, acting actively in the decomposition process and used as a bioindicator group of environmental conditions. Our work tries to fill some gaps that still exist in the collecting methodology's standardization and effectiveness in the Amazon region, which has great biodiversity but is hugely threatened by human activity. Our results showed that Mesembrinellids family are more generalist and can be collected similarly regardless of the resource or trap used. But Calliphorid's family showed a difference in effectiveness depending on the resources and traps used. Thus, directly influencing the effectiveness of capturing the existing biodiversity and consequent on the interpretation of the inventory data and monitoring of areas.

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27- Toxic tests show the sensitivity of the Daphnia similis (Crustacea, Cladocera) and Pseudokirchneriella subcapitata (Chlorophyceae) to commercial pesticides

ADELINA M. KÜHL, EVALDO L.G. ESPÍNDOLA, TATIANE MANTOVANO, SABRINA DEOSTI & FÁBIO A. LANSAC-TÔHA

The present study aimed to assess the sensitivity of Daphnia similis Straus 1820 and Pseudokirchneriella subcapitata (Korshikov) F.Hindák to three commercial pesticides with different active ingredients. The compound most toxic to D. similis was the insecticide Dimilin® , followed by the fungicide Aproach Prima® and the herbicide Siptran 500SC®. In contrast, Siptran 500SC® was the most toxic compound to P. subcapitata, followed by Aproach Prima® and Dimilin®. The toxicity of the fungicide to algae and microcrustaceans demonstrates that compounds developed for certain organisms are able to affect others, indicating the relevance of conducting ecotoxicological tests on different organisms. 

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28- Structure and function of calls of the Masked Gnatcatcher Polioptila dumicola in Mid-eastern Argentina

EVELINA LEON, RODRIGO LORENZÓN, PAMELA OLGUÍN, ANDRES ATTADEMO, ADOLFO BELTZER & PAOLA PELTZER

Polioptila dumicola is a social bird species with great amplitude in its call repertoire. We found that adult males emitted at least 12 different calls that varied in structure and function. These calls were classified as alarm or contact depending on behavior of each individual. The wide variety of calls such as those found in P. dumicola may allow birds to distinguish different environmental and ecological situations. This study is the first for this species and provides a detailed description of the call repertoire that can be useful in future comparative studies, like those related to call variation and complexity among bird species.

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29- Insect galls from the Serra Negra do Funil Natural Heritage Private Reserve, Rio Preto, MG (Southeastern Brazil)

VALÉRIA C. MAIA & BERNARDO MASCARENHAS

Serra Negra do Funil Natural Heritage Private Reserve (NHPR), located in MG, was surveyed for insect galls.  A total of 63 gall morphotypes were found on 48 plant species of 22 families. Fabaceae and Asteraceae harbored the greatest gall richness. Twelve host plants are endemic to Brazil, one in vulnerable and one near threatened. Most galls were induced by Cecidomyiidae (Diptera). Parasitoids, cecidophages and successors were recorded. New plant-gall inducer associations were provided. The role of gall-inducers as ecosystem engineer was reinforced. The high number of endemism shows the importance of this NHPR for the biodiversity conservation. 

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30- Biofouling initial succession on offshore artificial substrate under subtropical conditions

VANESSA O. AGOSTINI & CARLA PENNA OZORIO

This study evaluated the contribution of depth, exposure time, UV-radiation, rainfall, temperature, pH, salinity, water chlorophyll-a, and zooplankton supply to initial marine macrofouling establishment on steel substrate exposed to the offshore subtropical environment. Not only meroplankton, but also tychoplankton and holoplankton, were recorded on the substrates, contributing to vagile fauna in macrofouling communities representing the higher species richness recorded on the substrates. This study showed that the depth of available substrates affects the macrofouling establishment, which is mainly associated with UV-radiation, exposure time, and ecological interspecific interactions. 

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GEOSCIENCES

31- Techniques for monthly rainfall regionalization in southwestern Colombia

TERESITA CANCHALA, CAMILO OCAMPO-MARULANDA, WILFREDO ALFONSO-MORALES, YESID CARVAJAL-ESCOBAR, WILMAR L. CERÓN & EDUARDO CAICEDO-BRAVO

Southwestern Colombia (Nariño) is a region with a complex topography and local factors that can influence the rainfall regime. Therefore, it is necessary to identify the best technique for monthly rainfall regionalization and define its spatial distribution and seasonality. This study evaluated four regionalization techniques where the best performance was presented for the approach related to non-linear principal component analysis and self-organizing map and indicated that Nariño has two regions with different rainfall regimes: the bimodal that dominates over the Andes mountains range and the unimodal in the coastal zone. These results are useful for water resource management. 

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32- Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin

KÁTIA S. TAKAYAMA, MARIANA C. MONTEIRO, PRISCILA SAITO, INGRID C. PINTO, CLAUDIA T. NAKANO, RENATA M. MARTINEZ, DOUGLAS V. THOMAZ, WALDICEU A. VERRI JR, MARCELA M. BARACAT, NILTON S. ARAKAWA, HELENA M. RUSSO, MARIA L. ZERAIK, RUBIA CASAGRANDE, RENÊ O. DO COUTO & SANDRA R. GEORGETTI

Brazilian’s contribution in the world´s market share of cosmetics is noteworthy. Rosemary (Rosmarinus officinalis L.) is a household spice displaying powerful antioxidant and anti-inflammatory properties. We have reported that a dermatological formulation (oil-in-water emulgel or gel cream) containing polyphenol-rich rosemary extract mitigated the skin damage following the exposure to UV-B radiation in an experimental animal model (pre-clinical trial). The sunscreen protection factor (SPF) that we have obtained met the Brazilian National Health Surveillance Agency (Anvisa) legislation for cosmetics. Our novel developing cosmeceutical may be an affordable sunscreen option for people worldwide concerned with skin care and longevity. 

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33- Holocene coastal evolution of Colorado River Delta based on diatom assemblages, Northern Patagonia, Argentina

ROCÍO FAYÓ, MARCELA ALCIRA ESPINOSA & FEDERICO IGNACIO ISLA

Diatoms are ubiquitous and abundant unicellular microalgae. After death, diatom valves are incorporated into sediments and their siliceous walls resist diagenetic degradation. They constitute an important fossil record and have been successfully used in Holocene paleoenvironmental reconstructions. Due to their sensitivity to salinity changes, diatoms represent one of the most used proxies to reinforce the interpretation of sedimentary facies and deltaic evolution based on eustatic changes. Diatom assemblages from a coastal sedimentary succession were studied at the deltaic plain of the Colorado River (Argentina) in order to reconstruct the environmental conditions in response to Holocene sea-level changes and delta progradation. 

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PALEONTOLOGY

34- Palaeoenvironmental changes based on foraminifera during the late Holocene at the Beagle Channel, Argentina

EMILIANA BERNASCONI, MARÍA S. CANDEL & ANA M. BORROMEI

This research is a contribution to the knowledge of the palaeoenvironmental conditions and palaeoclimatic changes during the late Holocene in the Beagle Channel using benthic foraminifera fauna, from a marine section of the Arroyo Baliza, located on the northwest coast of the Beagle Channel, as a proxy data. In this context, the integration of the foraminiferological study of a marine section from Arroyo Baliza (AB) at Bahía Lapataia, together with the palynological data provides new information to adjust the palaeoenvironmental reconstruction during the transgressive-regressive marine Holocene event in the Beagle Channel, southernmost Patagonia, Argentina. 

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ANIMAL SCIENCE

35- Helminth endoparasites of endemic fish Pygocentrus piraya (Characiformes, Serrasalmidae) from Três Marias reservoir, Minas Gerais, Brazil

MICHELLE D. SANTOS-CLAPP, RAYANE DUARTE, MARCIA C. ALBUQUERQUE & MARILIA C. BRASIL-SATO

This article highlights the endoparasitic fauna of the piranha Pygocentrus piraya, endemic species of the São Francisco River Basin. It’s a carnivorous fish that draws attention by your voracity, including accidents with humans. It’s fishing is considered because it is tasty and well appreciated meat, but their parasitism must be seen with care, as the piranhas has a broad spectrum of parasites, which can cause zoonoses by the accidental ingestion of these worms. In this way, Ichthyoparasitology constitutes an important tool in the diagnosis of pathogens agents that causes diseases in fish and humans. 

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36- Pathological changes by spirorchiid eggs in hawksbill sea turtle (Eretmochelys imbricata) stranded off brazilian coast

MARIA APARECIDA DA SILVA, RAPHAEL M. MEDINA, HASSAN J. LEANDRO, RACHEL B. RIBEIRO, RENATO LUIZ SILVEIRA, MARIAH B.R.G. PETRONILHA, MAX R. WERNECK, EDUARDO SHIMODA & EULÓGIO CARLOS Q. DE CARVALHO

Eretmochelys imbricata is classified as a critically endangered species. Spirorchiids are parasites of the circulatory system. The aim of the present study was to describe the pathology caused by spirorchiid eggs in E. imbricata from the Brazilian over a five years period. A total of 29 animals were analyzed, of which nine presented lesions. The lesions were: granulomatous enteritis in six, granulomatous splenitis in five, granulomatous hepatitis in three, granulomatous pneumonia in three, granulomatous pancreatitis in two, and granulomatous adrenalitis in one specimen. Concluded the main lesion in Eretmochelys imbricata was giant-cell granulomatous inflammatory reaction to the parasite's eggs. 

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37- Mechanisms of resistance and tolerance against parasites in fish: the impairments caused by Neoechinorhynchus buttnerae in Colossoma macropomum

JAQUELINE D. BILLER & EDSANDRA C. CHAGAS

Tambaqui fish farms deals with serious sanitary problems due to the prevalence of the Neoechinorhynchus buttnerae. However, the prevalence of the acanthocephalan parasite infections depends on the resistance and tolerance interactions between the host organisms and parasites. The defense against parasites is divided between innate and acquired immunity, the first is a result of physical barriers, cellular and humoral compounds, the second occurs through the antibodies production, and is mediated by cells, mainly by type 2 T helper lymphocytes. This specific mechanism is classified as classic type 2 response. The host-parasite relationship is complex and makes prevention and treatment difficult. 

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38- The scientific literature on bats (chiroptera) in Brazil: a scientometric analysis from 1954 – 2018

BEATRIZ FERNANDES L. LUCIANO, GUILHERME ALVES ELIAS, JAIRO JOSE ZOCCHE, ERALDO M. COSTA NETO & FERNANDO CARVALHO

The aim of this study was to analyze the literature on bats in Brazil, identifying patterns, tendencies and knowledge gaps in the Brazilian federal states. We carried out a sistematyzed search on the online databases WoS, SciELO, PubMed and Scopus. We used the descriptive terms “Chiroptera” and “Brazil”. Besides these bases, we included data from the manuscripts published in Chiroptera Neotropical. Even though the increasing in the number of articles by categories, we encourage that every approach keep being developded, once no thematic could had been considered enough explored till the moment. 

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39- Addition of açai oil during the close-up dry period of Holstein cows improves colostrum quality and immune responses of their calves

DAIANE S. DOS SANTOS, VANDERLEI KLAUCK, CLEITON THEISEN, BRUNA BORDIGON, RENAN FARINA, WANDERSON A.B. PEREIRA, CARINE F. SOUZA, MATHEUS D. BALDISSERA, ANA LUIZA B. SCHOGOR, MARCELO VEDOVATTO, ELIZABETH A. PALMER & ALEKSANDRO S. DA SILVA

The use of açai oil in the diet of prepartum cows had immunological and antioxidant properties. Cows fed açai oil produce colostrum with greater concentrations of immunoglobulins and antioxidant capacity. Cows fed açai oil produced colostrum with reduced lipoperoxidation and greater concentrations of immunoglobulin A. Calves fed with colostrum from cows that received açai oil had greater serum protein due to increased heavy chain IgG, IgA and α-lactalbumin in colostrum. The addition of 4% açai oil in the prepartum diet of cows may be an effective strategy to improve colostrum quality, and consequently calf health.   

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40- Physical and chemical characteristics of meat from lambs fed sorghum silage with cashew bagasse

HILTON F.M. BARRETO, ANA PAULA P. DE ASSIS, RENATA N. DE LIMA, ELAINE C.A. SOARES, ÊLIKA S. DE SOUSA, ANDREZZA A.C. MOURA, VITOR L.L. MELO, CICÍLIA M.S. DE SOUZA, JOÃO VIRGÍNIO E. NETO & PATRÍCIA O. LIMA

This study was carried out to evaluate the effects of replacing forage sorghum with dehydrated cashew bagasse (DCB) in the proportions of 0; 8; 16 and 24%, based on the natural matter at ensiling on the physical-chemical parameters of confined lambs meat, verifying that the replacement of forage sorghum up to 24% of the dehydrated cashew bagasse in the silage does not compromise the quality of meat, however, the substitution of 8% is preferred, as it provides meats with lower lipidic content.  

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41- Improving geographic distribution data for a putatively extinct species, a test case with a disappeared fly

PABLO R. MULIERI, SILVINA MIGALE, LUCIANO D. PATITUCCI, CHRISTIAN R. GONZÁLEZ & SARA I. MONTEMAYOR

The study is focused on a blowfly poorly known and presumed to be confined to southern-South America, with an unclear distributional pattern. It was last collected in 1984. We aimed, through Ecological Niche Models, to identify regions highly suitable; with high chances to find it; to test the suitability of regions with doubtful records; and to understand the impact that climatic change and human activities have had. Results indicate where suitable conditions are found, that doubtful records are in unsuitable areas, and that its decline is not mainly caused by climate changes, habitat loss and biological invasion.  

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42- The use of Enzymes in Meat Quail Diets Containing Sunflower Seed Cake

CARLA N. CORDEIRO, EDNARDO R. FREITAS, RAFAEL C. NEPOMUCENO, SARAH G. PINHEIRO, DAVYD H. SOUZA, EDIBERGUE O. SANTOS, MARCELLE C.A. DE MELO, ANNA KAYLLYNY O. SILVA & GERMANO A.J. DO NASCIMENTO

A technique used in poultry production to reduce feed costs is the use of alternative foods to replace the most used foods, corn and soybean meal. An alternative would be the use of sunflower seed cake, a by-product of biodiesel production. However, the concentration of fiber present in this food can impair the metabolism of nutrients, an effect that can be reduced with the use of enzymes. The results indicated that the use of enzymes in the diet meat quails improves energy metabolization and economic viability of rations containing sunflower seed cake. 

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43- Different proportions of starch and neutral detergent-soluble fiber in diets for feedlot lambs

JOSEMIR S. GONÇALVES, JANE M.B. EZEQUIEL, ANTÔNIO C. HOMEM JÚNIOR, FLAVIA O.S. VAN CLEEF, OTÁVIO R. MACHADO NETO & ERIC H.C.B. VAN CLEEF

This study was conducted to evaluate the replacement of corn (conventional starch-rich energy ingredient) by citrus pulp (an alternative soluble fiber-rich energy ingredient) in diets for feedlot finishing lambs. The animals were fed in feedlot for 84 days. The use of 250 g of the by-product/kg dry matter impaired performance (feed intake, body weight gain), carcass (hot weight, dressing percentage, weight of commercial cuts), and nitrogen balance (retained nitrogen in the body). However, the moderate replacement of corn by citrus pulp did not change productive variables. Further research is needed testing other soluble fiber sources for sheep. 

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44- Encapsulated nitrate replacing soybean meal in diets with and without monensin on in vitro ruminal fermentation

ANDRESSA S. NATEL, ADIBE LUIZ ABDALLA, RAFAEL C. DE ARAUJO, TIAGO P. PAIM, ADIBE LUIZ ABDALLA FILHO, PATRÍCIA LOUVANDINI, MELKI K. LIMA & PAOLA PIZA

Focusing on a nutritional alternative that manipulate rumen fermentation, this study assessed the association of encapsulated nitrate product (ENP) and monensin (MON) to mitigate enteric methane (CH4) in vitro and possible effects on fermentation characteristics and microbial populations. Six treatments were used with two levels of MON (0 and 2.08 mg/mL of buffered rumen fluid) and three levels of ENP (0, 1.5 and 3.0%).  ENP replaced soybean meal. The ENP addition decrease CH4 production without affecting  truly degraded organic matter nor the relative abundance of methanogens. However, no additive effect of ENP and MON was observed for mitigating CH4 production. 

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45- Maxiclavella and Praeclavella (Siphonostomatoida: Lernaeopodidae) new genera confirmed by molecular and morphological evidence

RAUL CASTRO-ROMERO, MARTIN MIGUEL MONTES & SERGIO MARTORELLI

The new genus Maxiclavella is proposed to accommodate Clavella simplex Castro Romero and Baeza Kuroki, 1985, which differs from Clavella species, including Praeclavella nasalis, new genus and species, was found parasitizing the olfactory sacs of Isacia conceptionis. other Clavella species by a biramous antenna with an exopod shorter than the endopod, Males of both genera fit well in the Clavella-clade male type, although they differ process, which is present in the latter and absent in the former. Also Clavella-branch (Clavellinae Wilson), a key based genera on female specimens is presented. 

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46- Nutritional performance, hepatic and renal function in goats fed diets containing detoxified castor cake at different stages of pregnancy

RICARDO A. DE ARAÚJO, JOSÉ N.M. NEIVA, MAGNO JOSÉ D. CÂNDIDO, MARCOS CLÁUDIO P. ROGÉRIO, LUIZA DE N.C. DA SILVA & ROBERTO CLÁUDIO F.F. POMPEU

The nutrition of goats during the gestation period is of fundamental importance in the production systems, implying in the production and composition of milk and in the weight gain of their offspring; The castor cake, when detoxified, can be an alternative source of protein for pregnant goats; During the final third of gestation, Saanen and Anglo Nubian goats reduce consumption considerably; Goats fed diets containing DCC NaOH have lower nutrient consumption due to higher sodium intake. However, the development of generated fetuses is not adversely affected; Goats fed diets containing DCC NaOH showed greater efficiency in the use of nitrogen. 

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47- Evaluation of methods for reducing epibionts during farming of the mangrove oyster Crassostrea gasar (Adanson, 1757)

ANTONIO OSTRENSKY, ALINE HORODESKY, GIORGI DAL PONT, NATHIELI COZER & GISELA G. CASTILHO-WESTPHAL

During cultivation, other organisms known as epibionts, compete with the oysters for food, space, oxygen and can negatively affect production. Thus, we assessed the efficacy of different methods used for the removal of epibionts from oyster shells during farming. At an oyster farm in South Brazil, we exposure oyster (Crassostrea gasar) to the following removing methods: freshwater; hypersaline water; sodium hypochlorite solution; quaternary ammonia solution; exposure to air; hydroblasting; and no cleaning procedure (control). After 15 and 30 days we measured total incrustation and mortality and the most efficient methods to reduce epibionts were freshwater exposure and hydroblasting. 

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48- Association between Ichthyocladius (Diptera: Chironomidae) and armored catfishes in the Guareí River basin, São Paulo State, Brazil

VALTER M. AZEVEDO-SANTOS, ERIKA M. SHIMABUKURO, FERNANDO M. PELICICE & RAOUL HENRY

Ichthyocladius is an insect genus whose immature forms live attached to the bodies of some species of freshwater fishes. In the manuscript published in Anais da Academia Brasileira de Ciências, we reported for the first time the species I. lilianae in the São Paulo State and in the Paraná River basin. Moreover, this is the first record of Ichthyocladius species associated with the armored catfishes (cascudos, in Portuguese) Hypostomus iheringi and H. tietensis. 

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49- Phosphorus release capacity in different dietary commercial phytases through performance and bone characteristics assessment of broiler chickens

ANTÔNIO G. BERTECHINI, MATHEUS P. REIS, FELIPE S. DALÓLIO & JULIO CESAR C. CARVALHO

Supplementation with commercial phytases in diets for broilers is a reality as it promotes the release of complexed phosphorus (P) in the phytic acid molecule in vegetables, in addition to providing minerals, amino acids and energy. However, the phytase releasing unit (FTU) has been used as a commodity to ensure adequate P release. Thus, the objective was to identify the P release capacity with phytase supplementation. The results of performance and bone characteristics indicated that there is a difference regarding the method of phytase evaluation and the ability to release P.  

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50- Evaluation of partial 12S rRNA, 16S rRNA, COI and Cytb gene sequence datasets for potential single DNA barcode for hylids (Anura: Hylidae)

RICARDO KOROIVA & DIEGO JOSÉ SANTANA

In this article, we evaluated the effectiveness of usual genetic methods for estimates of biodiversity and specimens’ identification in anurans. Coding genes, especially COI, had a better identification capacity than non-coding genes, such as 16S and 12S. These results guide the use of a single gene in studies to assess anuran biodiversity. Considering that amphibians are facing severe threats and the limitation of taxonomists and financial resources for conservation, we provide an indication for a better allocation of resources in genetic studies. 

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51- Changes on the physicochemical and fatty acid profile of meat induced by inclusion of biscuit bran in lamb diet

RENATA T. ALENCAR, WILDER H.O. VEGA, LUIZA N.C. DA SILVA, HÉLIO H.A. COSTA, MICHELLE O.M. PARENTE, LISIANE D. DE LIMA & ALINE V. LANDIM

This work seeks to give scientific validity to the feed management routine used empirically by producers with the use of co-products purchased at low cost, in this case, the biscuit bran. Furthermore, it intends to present a novel strategy for managing the energy resource in the diet of lambs, which increases the profit in sheep properties. Four years of follow-up to producers plus the time of the experiment are reported here. Metabolic aspects as well as carcass quality validate its use in regions where the co-product is available.

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52- Different protocols for piglet creep feeding in the farrowing stage and residual effects in the nursery phase

FERNANDO ZIMMER, GABRIELA M. GALLI, HIAM J. MARCON, DAVI F. ALBA, LUIZ GRISS, KEYSUKE MURAMATSU, ALEKSANDRO S. DA SILVA & DIOVANI PAIANO

The experiment was carried out under commercial farm conditions and evaluated different creep feeding methodologies for piglets and their effects on performance, serum variables at weaning and nursery phase. At weaning the treatments without supplement and additional liquid creep with automatic feed dispenser promoted higher piglet body weight as than to other creep feeding methodologies. There was no effect on performance in the nursery phase and on the third day after weaning the serum concentration of aspartate aminotransferase was higher in the treatment without prior supplementation.   

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CROP SCIENCE

53- Development and characterization of antioxidant and antimicrobial poly (butylene adipate-co-terephtalate) (PBAT) film incorporated with oregano essential oil and applied in sliced mozzarella cheese

LUCAS G. CARDOSO, JÂNIA B.A. DA SILVA, JULIANA A. DA SILVA, GEANY P. CAMILLOTO, CAROLINA O. DE SOUZA, JANICE I. DRUZIAN & ALAÍSE G. GUIMARÃES

Developed a biodegradable active film with Poly(butylene adipate-co-terephthalate) (PBAT) incorporated with oregano essential oil (OE). The intention is to minimize the environmental impacts caused by synthetic packaging and to provide new technologies through biodegradable polymers incorporated with natural antimicrobial. Contributing to food security and consequently safe human health, in addition to new technology for the food industry. It was possible to obtain films with low porosity, extend cheese shelf-life up to 35 days, and have good mechanical properties for the application potential of the film as active packaging at industrial scales. 

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54- Effect of the seed coating with biomass of Dunaliella salina on early plant growth and in the secondary metabolites content of Coriandrum sativum

YASMIN B.T. FONSECA, NICOLE M. ALMEIDA, JAMILE C. CALDAS, GABRIEL N. MORAIS, ISAAC M.J. SILVA, VALÉRIA B. RIATTO, WALTER N.L. SANTOS & FERNANDO L.B. MOUTINHO

To this day, we face the challenge of increasing crop productivity through sustainable and inclusive technologies, capable of using natural resources to benefit the environment and the lives of farmers. Therefore, the present research sought to develop a seed coating from starch biopolymer and microalgae biomass that sustainably increases both crop productivity and quality. The study results showed a promising performance, creating a positive perspective for meeting the challenges of a sustainable and accessible agriculture. 

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55- Assessing the carbon stock of cultivated pastures in Rondônia, southwestern Brazilian Amazon

CARLOS R. SANQUETTA, ALEXIS DE S. BASTOS, MATEUS N.I. SANQUETTA, IARA M. BARBERENA, ANA P. DALLA CORTE, ALEXANDRE QUEIROZ & LUIZ FELIPE P.U. ALMEIDA

Cattle ranching is the primary land-use of deforested areas in the Brazilian Amazon. Deforestation precedes pasture establishment, implying tremendous amounts of greenhouse gas emissions to the atmosphere. Despite several studies addressing carbon storage in forests, there is a lack of data regarding cultivated pastures. In this study, we assessed the carbon stock of cultivated pastures located in the southwestern Brazilian Amazon. Our results indicated that land-use change from primary forests to cultivated pastures resulted in a loss of 192.54 MgC.ha^-1, corresponding to a net emission of 705.98 MgCO2eq.ha^-1.  

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56- Sequential sampling plan for Ceratitis capitata (Diptera: Tephritidae) in guava orchards

JOSÉ NICÁCIO, ALFREDO R. ABOT, MARIANA P. OLIVEIRA, JAINE L. SILVA & FLÁVIO R.M. GARCIA

The objectives of this research were: (i) to Estimate the quantitative occurrence of Ceratitis capitata captured in McPhail traps in cultivating guava; (ii) to investigate the spatial distribution patterns of C. capitata in guava orchards; (iii) to compare the index of the FTD (fruit fly/trap/day) to the type of spatial distribution of  C. capitata with the Negative Binomial to set the best time for control of the population. C. capitata has aggregated distribution behavior. The potential risk of economic damage is associated with aggregate distribution behavior. The beginning of this distribution indicates the most appropriate time for using control techniques. 

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57- Site classification for Eucalyptus sp. in a tropical region of Brazil ATAÍDES M. FILHO,

ATAÍDES M. FILHO, SYLVIO P. NETTO, SEBASTIÃO A. MACHADO, ANA P.D. CORTE & ALEXANDRE BEHLING

Site index is strongly correlated to growth factors and volume yield. It is also influenced by density and treatments like thinning. This research was conducted to generate productivity classes, applying three methods (GC, ADA, and GADA) to estimate site curves for Eucalyptus sp. in a tropical region of Brazil, using permanent sample plots annually measured between 2011–2014, and the application of geostatistics to classify the cartographic bases of the plantations. GADA approach provided the best statistical  results with improvement of 12,23%  and 39,80% on the ADA and GC methods, respectively. 

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58- Recovery of bioactive compounds from an agro-industrial waste: extraction, microencapsulation, and characterization of jaboticaba (Myrciaria cauliflora Berg) pomace as a source of antioxidant

SUELEN S. DOS SANTOS, CAROLINA M. PARAÍSO, SILVIO CLÁUDIO DA COSTA, CAMILLA YARA L. OGAWA, FRANCIELLE SATO & GRASIELE S. MADRONA

This study aimed to evaluate the extraction of bioactive compounds from jaboticaba pomace, produce microcapsules by spray dryer technique, and characterize antioxidant compounds. A factorial experimental design was used in the extraction step. Maltodextrin (DE 10) was used as an encapsulating agent, in a ratio of 1: 1 (w/w), in the microencapsulation process. It was observed the increase of all bioactive compounds analyses comparing jaboticaba pomace with the extract. ATR-FTIR spectroscopy showed a vibrational stretching aromatic ring (1718 – 1731 cm-1) typical for anthocyanins. The Gaussian deconvolution presented extract peak area 7.56% higher than pomace. The encapsulating agent protected anthocyanins during the drying process. Microencapsulation of bioactive compounds from jaboticaba pomace can be useful for food applications whereas they are a rich source of antioxidant compounds. Moreover, the use of agro-industrial waste is promising linked to the use of clean technology as water as an antioxidant extractor. 

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SOIL SCIENCE

59- Modelling soil water retention to identify management influence on soil pore characteristics

RODRIGO F. DA SILVA, ERNANDES G. MOURA, GILBERTO R. LISKA, GERALDO C. DE OLIVEIRA, DANIEL F. FERREIRA, MOACIR S. DIAS JUNIOR & GLENIO G. SANTOS

Water is essential for plants and animals. In soil, the dynamics of water is associated with the forces of cohesion and adhesion to solid particles and the organization of these particles in a porous structure. Soil water storage can be described using the water retention curve. Management influences the porous structure of the soil, which in turn modifies the availability of water to plants. In this sense, the present study comes up with a proposal to use statistical techniques to compare and choose the most appropriate mathematical models in the description of water storage for each type of soil management. 

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ENGINEERING SCIENCES

60- Eutrophication risk assessment of a large reservoir in the Brazilian semiarid region under climate change scenarios

JOÃO B.S. RAULINO, CLEITON S. SILVEIRA & IRAN E.L. NETO

This study assesses the risk of eutrophication of a large semiarid reservoir under SSP2-4.5 and SSP5-8.5 scenarios and different conditions of inlet total phosphorus (TP) concentration and reservoir withdrawal. The results suggest that the end of the 21st century will experience the highest impacts on water quality. On the other hand, major reductions in TP and reservoir withdrawal are necessary to improve the trophic status and minimize the risk of eutrophication. The results also showed that the dry period is more susceptible to eutrophication than the rainy period and that semiarid reservoirs are highly vulnerable to eutrophication under climate change. 

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61- Temperature pull-down of a retrofitted wine refrigerator cabinet cooled by a caloric system emulator

RICARDO S. CALOMENO, SERGIO L. DUTRA, NATÁLIA M. DE SÁ, GUILHERME F. PEIXER, JAIME A. LOZANO & JADER R. BARBOSA JR

Magnetic refrigeration is an alternative cooling technology based on the magnetocaloric effect, defined as the thermal response of some materials to changes in the applied magnetic field. This study focuses on a particular stage of the design of a compact magnetic wine cooler, namely the development and validation of a mathematical model to simulate the time-dependent thermal behavior of a retrofitted insulated cabinet filled with bottles and driven by a caloric system emulator. In addition to the good agreement with the experimental cooling capacity, the numerical model quantified the thermal losses through the glass door and external cabinet surface area. 

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62- Empirical and semi-empirical chlorophyll-a modeling for water quality assessment through river-lake transition in extreme Southern Brazil

CASSIA B. CABALLERO, HUGO ALEXANDRE S. GUEDES, ALICE CÉSAR F. DE ANDRADE, VITOR S. MARTINS, ROSIMÉRI S. FRAGA & KAREN G.P. MENDES

Empirical chlorophyll-a (Chl-a) modeling using remote sensing and in situ data for water quality assessment was performed in the Mirim lagoon, Southern Brazil. Water quality data were collected simultaneously with Sentinel-2 satellite overpass. Low Chl-a concentration is observed at the Northern lagoon, possibly due to suspended solids presence. The same occurs in the left margin, being associated with the influence of land use for agriculture. The mean Chl-a concentration predicted by the model was similar to the in situ value. The model developed is tool to reduce costs and efforts in fieldwork measures, understanding eutrophication in this river-lake transition ecosystem.

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63- Correlating blast vibrations and geomechanical properties to determine damage profiles and improve wall conditions in open pit mining

GUSTAVO S. LOPES, GERMAN VINUEZA, BARBARA TRZASKOS, AUGUSTO F. RIBEIRO & RAFAEL G. ARAUJO

Slope stability is one of the biggest concerns for mining practices today due to the safety hazards and financial impacts on mineral extraction. Considering the rock mass response to rock blasting is essential to achieve the desired pit geometry and provide an optimized and safe mineral exploration. The central element of this study was the construction of a model which, considering geomechanical properties, allowed the characterization of this particular rock mass, understanding the generation of damage caused by explosives and optimizing the mining practices. 

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64- Efficiency of renewable energy plants in Russia

ALEXEY MIKHAYLOV

This research paper's comprehensive evaluation of the effectiveness of Russian power plants' techno-economic processes and indicators serves as the foundation for the formulation of recommendations for their effective application and improvement. The payback period for SWH plants using a drain-down system is 5–7 years, and capital costs are 1,4–1,6 times lower than for standard SWHs with circulation systems. Costs for biogas produced in specialist medium- and high-power plants range from $15,000 to 60,000 per cubic meter. However, biogas generated in low-energy power plants costs between $80,000 and $270,000, which is less than natural gas. Therefore, at this time, using biogas is economically viable. 

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65- Effect of blueberry addition on antioxidant activity, textural, microbiological and physicochemical properties of strained yoghurt

MUSTAFA ŞENGÜL, BÜŞRA CAN, BAYRAM ÜRKEK & ZEYNEP GÜRBÜZ-KAÇAN

Fermented dairy products have many positive effects on human health and are consumed at worldwide. Different ingredients are added for increasing the beneficial effects of the products, and research has increased regarding functional dairy, recently. Yoghurt is one of the important fermented dairy products. The study is on improving the functional properties of strained yoghurt added blueberry. 

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66- An approach to robust condition monitoring in industrial processes using pythagorean membership grades

ADRIÁN R. RAMOS, JOSÉ M.B. DE LÁZARO, CARLOS C. CORONA, ANTÔNIO J. DA SILVA NETO & ORESTES LLANES-SANTIAGO

Modern industry must maintain high levels of productivity and efficiency. Faults in the industrial systems affect those goals. In order to reduce the effect of faults, reliable early fault detection and location systems need to be installed in the industry. This paper proposes a system for detecting and locating faults in the industry based on advanced computational intelligence tools, specifically fuzzy logic, which achieves high-performance rates. The proposal is validated using experimental datasets and the Tennessee Eastman (TE) process benchmark. The results are compared with the results obtained with other algorithms present in the scientific literature showing the advantages of the made proposal.  

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SOCIAL SCIENCES

67- Science communication activities at research institutes in Brazil: Recent expansion on the way to professionalization

LUISA MASSARANI, CARLA M. DA SILVA, LUIS AMORIM & DIRLENE M.S. MARIA

A study published at the Annals of the Brazilian Academy of Science by Massarani and collaborators suggest that the last five years have seen an increase in science communication by research institutes responsible for a wide range of activities. To communicate with the public, the 169 institutes studied tend to use traditional communication channels more than social media. However, most of them did not have designated communication teams, drawing on specialized personnel from their host institution. Although they do engage in intensive communication activities, Brazilian research institutes still lack more a structured approach to science communication.  

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All abstracts of the publications were provided by the respective authors.

Annals of the Brazilian Academy of Sciences
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